Differential expression patterns of two cellulose synthase genes are associated with primary and secondary cell wall development in aspen trees.
Identifieur interne : 004288 ( Main/Exploration ); précédent : 004287; suivant : 004289Differential expression patterns of two cellulose synthase genes are associated with primary and secondary cell wall development in aspen trees.
Auteurs : Udaya C. Kalluri [États-Unis] ; Chandrashekhar P. JoshiSource :
- Planta [ 0032-0935 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN complémentaire (isolement et purification), ADN des plantes (génétique), ADN des plantes (isolement et purification), ARN messager (génétique), Amorces ADN (MeSH), Arbres (croissance et développement), Arbres (enzymologie), Arbres (génétique), Hybridation d'acides nucléiques (MeSH), Paroi cellulaire (physiologie), Populus (croissance et développement), Populus (enzymologie), Populus (génétique), RT-PCR (MeSH), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes végétaux (MeSH).
- MESH :
- croissance et développement : Arbres, Populus.
- enzymologie : Arbres, Populus.
- génétique : ADN complémentaire, ADN des plantes, ARN messager, Arbres, Populus.
- isolement et purification : ADN complémentaire, ADN des plantes.
- physiologie : Paroi cellulaire.
- Amorces ADN, Hybridation d'acides nucléiques, RT-PCR, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Cell Wall (physiology), DNA Primers (MeSH), DNA, Complementary (genetics), DNA, Complementary (isolation & purification), DNA, Plant (genetics), DNA, Plant (isolation & purification), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Plant (MeSH), Nucleic Acid Hybridization (MeSH), Populus (enzymology), Populus (genetics), Populus (growth & development), RNA, Messenger (genetics), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Trees (enzymology), Trees (genetics), Trees (growth & development).
- MESH :
- chemical , genetics : DNA, Complementary, DNA, Plant, RNA, Messenger.
- chemical , isolation & purification : DNA, Complementary, DNA, Plant.
- chemical : DNA Primers.
- enzymology : Populus, Trees.
- genetics : Populus, Trees.
- growth & development : Populus, Trees.
- physiology : Cell Wall.
- Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Nucleic Acid Hybridization, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
The quality and quantity of cellulose deposited in the primary and secondary cell walls of plants vary in accordance with their biological function. However, the molecular basis of such cellulose heterogeneity has so far remained unclear. Since enrichment of better-quality cellulose, in terms of increased degree of polymerization and crystallinity, is one of the goals of forest biotechnology, our main objective is to decipher the roles of distinct cellulose synthase (CesA) genes in tree development, with special reference to wood production. Here, we report two full-length CesA cDNAs, PtrCesA3 and PtrCesA4, from an economically important tree aspen (Populus tremuloides). PtrCesA3 is orthologous to the Arabidopsis AtCesA4 gene involved in secondary wall formation, whereas PtrCesA4 is orthologous to the Arabidopsis AtCesA1 gene involved in primary cell wall formation. To define the specific cell types expressing these CesA genes, we explored the natural distribution patterns of PtrCesA3 and PtrCesA4 transcripts in a variety of aspen organs, such as leaves, petiole, stem, and roots, using in situ hybridization with hypervariable region-specific antisense riboprobes. Such a side-by-side comparison suggested that PtrCesA3 is exclusively expressed in secondary-wall-forming cells of xylem and phloem fibers, whereas PtrCesA4 is predominantly expressed in primary-wall-forming expanding cells in all aspen organs examined. These findings suggest a functionally distinct role for each of these two types of PtrCesAs during primary and secondary wall biogenesis in aspen trees, and that such functional distinction appears to be conserved between annual herbaceous plants and perennial trees.
DOI: 10.1007/s00425-004-1329-z
PubMed: 15278458
Affiliations:
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Kalluri</name><affiliation wicri:level="2"><nlm:affiliation>Plant Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Joshi, Chandrashekhar P" sort="Joshi, Chandrashekhar P" uniqKey="Joshi C" first="Chandrashekhar P" last="Joshi">Chandrashekhar P. Joshi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15278458</idno><idno type="pmid">15278458</idno><idno type="doi">10.1007/s00425-004-1329-z</idno><idno type="wicri:Area/Main/Corpus">004234</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004234</idno><idno type="wicri:Area/Main/Curation">004234</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004234</idno><idno type="wicri:Area/Main/Exploration">004234</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Differential expression patterns of two cellulose synthase genes are associated with primary and secondary cell wall development in aspen trees.</title><author><name sortKey="Kalluri, Udaya C" sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name><affiliation wicri:level="2"><nlm:affiliation>Plant Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Plant Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Joshi, Chandrashekhar P" sort="Joshi, Chandrashekhar P" uniqKey="Joshi C" first="Chandrashekhar P" last="Joshi">Chandrashekhar P. Joshi</name></author></analytic><series><title level="j">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (physiology)</term><term>DNA Primers (MeSH)</term><term>DNA, Complementary (genetics)</term><term>DNA, Complementary (isolation & purification)</term><term>DNA, Plant (genetics)</term><term>DNA, Plant (isolation & purification)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Nucleic Acid Hybridization (MeSH)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>RNA, Messenger (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Trees (enzymology)</term><term>Trees (genetics)</term><term>Trees (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN complémentaire (isolement et purification)</term><term>ADN des plantes (génétique)</term><term>ADN des plantes (isolement et purification)</term><term>ARN messager (génétique)</term><term>Amorces ADN (MeSH)</term><term>Arbres (croissance et développement)</term><term>Arbres (enzymologie)</term><term>Arbres (génétique)</term><term>Hybridation d'acides nucléiques (MeSH)</term><term>Paroi cellulaire (physiologie)</term><term>Populus (croissance et développement)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>RT-PCR (MeSH)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>DNA, Plant</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ADN des plantes</term><term>ARN messager</term><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN complémentaire</term><term>ADN des plantes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Nucleic Acid Hybridization</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Hybridation d'acides nucléiques</term><term>RT-PCR</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The quality and quantity of cellulose deposited in the primary and secondary cell walls of plants vary in accordance with their biological function. However, the molecular basis of such cellulose heterogeneity has so far remained unclear. Since enrichment of better-quality cellulose, in terms of increased degree of polymerization and crystallinity, is one of the goals of forest biotechnology, our main objective is to decipher the roles of distinct cellulose synthase (CesA) genes in tree development, with special reference to wood production. Here, we report two full-length CesA cDNAs, PtrCesA3 and PtrCesA4, from an economically important tree aspen (Populus tremuloides). PtrCesA3 is orthologous to the Arabidopsis AtCesA4 gene involved in secondary wall formation, whereas PtrCesA4 is orthologous to the Arabidopsis AtCesA1 gene involved in primary cell wall formation. To define the specific cell types expressing these CesA genes, we explored the natural distribution patterns of PtrCesA3 and PtrCesA4 transcripts in a variety of aspen organs, such as leaves, petiole, stem, and roots, using in situ hybridization with hypervariable region-specific antisense riboprobes. Such a side-by-side comparison suggested that PtrCesA3 is exclusively expressed in secondary-wall-forming cells of xylem and phloem fibers, whereas PtrCesA4 is predominantly expressed in primary-wall-forming expanding cells in all aspen organs examined. These findings suggest a functionally distinct role for each of these two types of PtrCesAs during primary and secondary wall biogenesis in aspen trees, and that such functional distinction appears to be conserved between annual herbaceous plants and perennial trees.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15278458</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0935</ISSN><JournalIssue CitedMedium="Print"><Volume>220</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Differential expression patterns of two cellulose synthase genes are associated with primary and secondary cell wall development in aspen trees.</ArticleTitle><Pagination><MedlinePgn>47-55</MedlinePgn></Pagination><Abstract><AbstractText>The quality and quantity of cellulose deposited in the primary and secondary cell walls of plants vary in accordance with their biological function. However, the molecular basis of such cellulose heterogeneity has so far remained unclear. Since enrichment of better-quality cellulose, in terms of increased degree of polymerization and crystallinity, is one of the goals of forest biotechnology, our main objective is to decipher the roles of distinct cellulose synthase (CesA) genes in tree development, with special reference to wood production. Here, we report two full-length CesA cDNAs, PtrCesA3 and PtrCesA4, from an economically important tree aspen (Populus tremuloides). PtrCesA3 is orthologous to the Arabidopsis AtCesA4 gene involved in secondary wall formation, whereas PtrCesA4 is orthologous to the Arabidopsis AtCesA1 gene involved in primary cell wall formation. To define the specific cell types expressing these CesA genes, we explored the natural distribution patterns of PtrCesA3 and PtrCesA4 transcripts in a variety of aspen organs, such as leaves, petiole, stem, and roots, using in situ hybridization with hypervariable region-specific antisense riboprobes. Such a side-by-side comparison suggested that PtrCesA3 is exclusively expressed in secondary-wall-forming cells of xylem and phloem fibers, whereas PtrCesA4 is predominantly expressed in primary-wall-forming expanding cells in all aspen organs examined. These findings suggest a functionally distinct role for each of these two types of PtrCesAs during primary and secondary wall biogenesis in aspen trees, and that such functional distinction appears to be conserved between annual herbaceous plants and perennial trees.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kalluri</LastName><ForeName>Udaya C</ForeName><Initials>UC</Initials><AffiliationInfo><Affiliation>Plant Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Joshi</LastName><ForeName>Chandrashekhar P</ForeName><Initials>CP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2004</Year><Month>07</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName 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development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>01</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>05</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>7</Month><Day>28</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>5</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>7</Month><Day>28</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15278458</ArticleId><ArticleId IdType="doi">10.1007/s00425-004-1329-z</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1450-5</Citation><ArticleIdList><ArticleId 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Joshi</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Kalluri, Udaya C" sort="Kalluri, Udaya C" uniqKey="Kalluri U" first="Udaya C" last="Kalluri">Udaya C. Kalluri</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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